Ascorbic Acid-Erythrocyte Interaction: ESR Investigations

نویسندگان

  • W. Lohmann
  • J. Schreiber
  • D. Holz
  • F. R. Hopkins
چکیده

A ddition o f alcohol oxidase to erythrocytes trea ted with ascorbic acid reverses the vitam in C effect, as expressed by the appearance of the sem idehydroascorbate signal, and the increase in spin concentration. The original erythrocyte electron spin resonance (ESR) signal is, how ever, never restored completely, as it is in the case if ascorbate oxidase is used. Additional interaction between the enzym e alcohol oxidase and haem oglobin iron m ust occur, since both of the iron ESR signals at g = 4.3 and g = 6 d isap p ear and the erythrocyte ESR spectrum at g ^ 2 is changed charac­ teristically. The spin concentration of the la tter spectrum increases again above a certain concentration o f alcohol oxidase.

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تاریخ انتشار 2013